Tuesday, April 15, 2014

Information on Gel Electrophoresis Lab: Due Tues, 4/22

Data Processing Part (done in class on Tues 4/15)
1)  Make Data Table for Ladder, Crime Scene, Suspect 1, Suspect 2, Suspect 3 to show distance traveled  of each band and then converted into DNA size in kilobases
2)  Measure practice gel bands (distance traveled from well in mm)
3)  Make graph on semi-log paper of Ladder information (Band 1 = 23,130 Kb, Band 2= 9,416 Kb, Band 3=6,557 Kb, Band 4=4,361 Kb, Band 5=2322 Kb, Band 6=2027 Kb).  Draw a distinct line of best fit that goes through the points as best as possible (should be curved)
4)  Use the graph, to complete the data table (convert CS, S1, S2, and S3 distance traveled to kilobases)

What you need to turn in on Tues 4/22:
a)  Completed lab hand-out (questions on first page and last page)
b)  Completed data table, neat and labeled graph, and practice gel photo taped to either.
c)  Court testimony:  1.5-2 pages on "who done it" including how gel electrophoresis works, your evidence, and possible sources of error and uncertainty.  You may use either your group's gel photo or the one I gave you.  If you want a JPG file of your group's photo, ask the group member who has it or myself and I can e-mail it to you.  Remember that you are an expert witness for the prosecution in a murder trial, so you may play the part as you see fit!

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